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PeproTech rhil-21
Rhil 21, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil-+21/rhil+21/pmc11556684-94-39-42
Average 90 stars, based on 1 article reviews
rhil-21 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Recombinant:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).

FACS:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).

Isolation:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).

Transfection:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).

Cytotoxicity Assay:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).

Generated:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).

Transduction:

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants
Article Snippet: Single CD19 + CD2 - CD4 - CD10 - CD14 - IgD - IgG + PvDBPII-TH2 + B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Atypical memory B cells from natural malaria infection produced broadly neutralizing antibodies against Plasmodium vivax variants.
Article Snippet: Single CD19+CD2-CD4-CD10-CD14-IgD-IgG+PvDBPII-TH2+ B cells were sorted onto pre-cultured MS40L-low feeder cells in 96 F plates containing RPMI 1640 medium supplemented with 10% FBS, 55 μM 2-mercaptoethanol (2-ME), penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM HEPES, 1 mM sodium pyruvate, 1% minimal essential medium non-essential amino acids, recombinant human interleukin-2 (IL-2; 50 ng/mL; PeproTech), recombinant human IL-4 (10 ng/mL; PeproTech), recombinant human IL-21 (10 ng/mL; PeproTech), recombinant human B-cell activating factor belonging to the TNF family (BAFF) (10 ng/mL, PeproTech) using FACS Symphony S6 (BD Biosciences).

Article Title: Generation of primary feline chimeric antigen receptor T cells.
Article Snippet: 1 X 105 enriched T cells/well were seeded in 100 μL of complete XVIVO-15 in 96-well round-bottom tissue culture plates (Thermo) with 10 ng/mL rfIL-2 (R&D Systems) alone or in combination with 100 ng/mL recombinant human IL-21 (rhIL-21; Peprotech), 5 μg/ mL ConA (Sigma), or 25 ng/mL PMA (Sigma) + 1 μg/ mL ionomycin (Sigma) for 24 hours.

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: B cell receptor dependent enhancement of dengue virus infection
Article Snippet: Both 7B9 and 2F3 cells were generated by transduction of IgM–CD27+ memory B cells with a retrovirus encoding human B cell lymphoma (BCL)-6 and BCL-xL and enhanced green fluorescent protein (GFP) and culture on CD40L-expressing L cell fibroblasts and rhIL-21 (25 ng/mL, Peprotech) as described [ ].

Article Title: Systemic tumor regression with synergy therapy: radiotherapy and CAR-T.
Article Snippet: The agents used in this study included the following: recombinant human interleukin-7/15/21 (PeproTech, Rocky Hill, NJ, USA), the EasySepTM Human T-Cell Isolation Kit (Stemcell, Vancouver BC, Canada), the ImmunoCultTM Human CD3/CD28/CD2 T-Cell Activator (Stemcell, Vancouver BC, Canada), the MojoSortTM Mouse CD3 T-Cell Isolation Kit (Biolegend, San Diego, CA, USA), the Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher, Waltham, MA, USA), the GeneJuice® Transfection Reagent (Merck, Rahway, NJ, USA), GlutaMAXTM (Gibco, GrandIsland, NY, USA), β-mercaptoethanol (Gibco, GrandIsland, NY, USA), polybrene (Sigma, St.Louis, MO, USA), puromycin (Sigma, St.Louis, MO, USA), the recombinant fibronectin fragment (Retronectin; Takara Shuzo, Japan), and the CytoTox 96® NonRadioactive Cytotoxicity Assay (Promega, Madison, WI, USA).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery.
Article Snippet: The cells were resuspended in RPMI-1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL−1 animal-free recombinant human IL-2 (PeproTech, USA), 100 μg mL−1 animal-free recombinant human IL-18 (MBL International, USA), and 10 μg mL−1 animal-free recombinant human IL-21 (PeproTech).

Article Title: Advancing Allogeneic NK Cell Immunotherapy through Microfluidic Gene Delivery
Article Snippet: The cells were resuspended in RPMI‐1640 medium (Corning, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, USA), 1% penicillin‐streptomycin (Gibco, Thermo Fisher Scientific, USA), 5 mM L‐glutamine (Gibco, Thermo Fisher Scientific), 10 IU mL −1 animal‐free recombinant human IL‐2 (PeproTech, USA), 100 μg mL −1 animal‐free recombinant human IL‐18 (MBL International, USA), and 10 μg mL −1 animal‐free recombinant human IL‐21 (PeproTech).



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